LOCUS       40924_23169       11285 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB513 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S.
  TITLE     Improved Gateway Binary Vectors: High-Performance Vectors for 
            Creation of Fusion Constructs in Transgenic Analysis of Plants
  JOURNAL   Biosci. Biotechnol. Biochem. (2007) In press
  PUBMED    17690442
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   Nakagawa T, Ishiguro S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, 
            Center for Integrated Research in Science; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan 
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, 
            Fax:81-852-32-6109)
REFERENCE   3  (bases 1 to 11285)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11285)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem. (2007) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, Center for 
            Integrated Research in Science"; volume: " 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan 
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, Fax"; 
            pages: "81-852-32-6109"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2083..2172
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      2206..2458
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2492..2508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2516..2532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2540..2570)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2585..2606)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2642..2894)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3048..4070)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4122..4301)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4807..4831)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5352..6140
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6389..6977
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7163..7303)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7647..7841)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7910..8980)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9412..10038)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcgggtta attaacatct tttacccata cgatgttcct
     2101 gactatgcgg gctatccgta tgacgtcccg gactatgcag gatcctatcc atatgacgtt
     2161 ccagattacg ctgctcagtg cagcgcttag agctcgaatt tccccgatcg ttcaaacatt
     2221 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     2281 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     2341 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     2401 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     2461 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2521 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2581 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tattaattcc
     2641 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     2701 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     2761 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     2821 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     2881 atgtttgaac gatcggggaa attcggggga tcgatcccgc gaactgtgga cgagaactgt
     2941 gccaccaagc gtaaggccgt tctctcgcat ttgccttgct aggctcgcgc gagttgctgg
     3001 ctgaggcgtt ctcgaaatca gctcttgttc ggtcggcatc tactctattc ctttgccctc
     3061 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     3121 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     3181 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     3241 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct
     3301 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     3361 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     3421 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     3481 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     3541 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     3601 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     3661 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     3721 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
     3781 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaatgcccc aatgtcaagc
     3841 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
     3901 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
     3961 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
     4021 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atcttattgc
     4081 cccccgggat cagatctgga ttgagagtga atatgagact ctaattggat accgagggga
     4141 atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct
     4201 taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc
     4261 cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
     4321 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat
     4381 gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     4441 cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
     4501 cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
     4561 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     4621 caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct
     4681 gagtgcttgc ggcagcgtga agctgatccg tcgagattaa tagaaataaa ttcagcctaa
     4741 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     4801 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     4861 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     4921 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     4981 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     5041 cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct
     5101 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     5161 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     5221 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     5281 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     5341 agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     5401 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     5461 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     5521 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     5581 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     5641 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     5701 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     5761 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     5821 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     5881 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     5941 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     6001 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     6061 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     6121 tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc
     6181 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     6241 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     6301 gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6661 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6721 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6781 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6841 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6901 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6961 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7021 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7081 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7141 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7201 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7261 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     7321 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7381 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     7441 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     7501 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     7561 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     7621 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     7681 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     7741 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     7801 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac
     7861 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     7921 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     7981 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     8041 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     8101 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     8161 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     8221 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     8281 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     8341 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     8401 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     8461 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     8521 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     8581 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     8641 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     8701 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     8761 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     8821 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     8881 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     8941 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
     9001 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc
     9061 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
     9121 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
     9181 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
     9241 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
     9301 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
     9361 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
     9421 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
     9481 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
     9541 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
     9601 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
     9661 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
     9721 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
     9781 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
     9841 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
     9901 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
     9961 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
    10021 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
    10081 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
    10141 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
    10201 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
    10261 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
    10321 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
    10381 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
    10441 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
    10501 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
    10561 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
    10621 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
    10681 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
    10741 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
    10801 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
    10861 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
    10921 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
    10981 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
    11041 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
    11101 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
    11161 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
    11221 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    11281 aacct
//
